STEP 511: Bacterial Inhibition Assay for Early Detection of Maple Syrup Urine Disease: Application for Neonatal Screening (CE: 1.0)


Maple Syrup Urine Disease (MSUD), also called branched-chain ketoaciduria, is an autosomal recessive metabolic disorder affecting branched-chain amino acids (BCAAs). Screening is conducted in the Philippines to detect MSU in newborns. This article discusses experimental research utilizing bacterial inhibition assay, specifically Escherichia coli MCC-MNH 1958, to detect excess BCAAs.


Learning Objectives:

  • Describe bacterial inhibition assay and the procedure
  • Explain the uses of the bacterial inhibition assay
  • Discuss the potential of Escherichia coli to detect Maple Syrup Urine Disease


Jon Paul M. Reyes, PhD, RMT, AHI (AMT), MSMLS, MBA, CHRA, DPed
Oliver Shane R. Dumaoal, RMT, MSMT, CBO, IFBA PC


Launched: 2023